Postdoc position in Cell Fate Reprogramming and Single-cell Biology

Embryonic Development
Engineering cell fate
Research in the Morris Lab
Postdoc position in Cell Fate Reprogramming and Single-cell Biology

The Morris Lab is a Stem Cell and Developmental Biology lab located in the Departments of Developmental Biology, and Genetics, and Center of Regenerative Medicine at the Washington University School of Medicine, Missouri, USA. Our research centers on the study of gene regulatory networks to dissect and engineer cell fate of clinically relevant tissues such as the intestine and liver. This focus integrates three major themes: First, we aim to understand how transcription factor overexpression drives changes in the transcriptional program to remodel cell identity, and how we can exploit this to derive desired cell types. Second, we transplant engineered cells into the in vivo niche, tracking their maturation in order to understand the steps required to fully differentiate cells in vitro. Finally, we employ single-cell transcriptomics to understand how cell fate is specified via reprogramming and in the developing embryo, formulating a blueprint of cell identity to help engineer fate in vitro. Ultimately, we wish to translate new insights in cell fate specification into better human models of disease and eventually into the development of novel therapeutic strategies.

As a member of our laboratory, you will be part of an interdisciplinary team responsible for conducting experiments focusing on the single-cell analysis of direct lineage reprogramming of fibroblasts to endoderm progenitors. You will be dissecting the mechanism of fate conversion using a combination of single-cell RNA-sequencing, cell biology, imaging, FACS, and transplantation assays.

Your tasks will be:
– Transcription factor mediated reprogramming of fibroblasts to endodermal fate
– High-throughput single-cell RNA-sequencing (Drop-seq) analysis of cell fate conversion and maturation. This includes microfluidic approaches to capture single-cells, and library preparation
In vitro assays and in vivo transplantation to assess maturation and function of engineered cells
– Data analysis

Your qualifications:
– PhD as cell biologist, stem/developmental biologist or molecular biologist required
– Strong knowledge in the areas of molecular biology, stem cell biology, RNA-sequencing
– Good experimental experience with cell culture, FACS, retro/lentiviral systems, RNA-sequencing
– Strong track record of scientific achievement (publications in peer reviewed journals)
– Excellent oral and written communication skills
– Strong team player with analytical skills and interest to work in a highly interdisciplinary area

Interested candidates should email Samantha Morris (s.morris[at]wustl.edu) for further details.